Pharma, dye & biochemical reagent intermediate
2,4-Dinitro Fluoro Benzene (2,4-DNFB) Supplier & Distributor in India & Worldwide
Chelora Agrochem is a trusted 2,4-Dinitro Fluoro Benzene (2,4-DNFB) supplier and distributor in India, connecting buyers with high-purity material sourced from leading, quality-approved manufacturers. Our 1-fluoro-2,4-dinitrobenzene — better known as Sanger's reagent — meets 99% min purity with low chloro-analogue content, full documentation and reliable supply across India and to buyers worldwide.
As a specialised distributor of chemical products, we do not manufacture in-house — instead we source only from reliable, audited manufacturers, giving you dependable material, competitive pricing and a single point of contact for all your 2,4-DNFB requirements, backed by COA, MSDS and TDS on every order.
- CAS number
- 70-34-8
- Formula
- C6H3FN2O4
- Molecular weight
- 186.10 g/mol
- Purity
- 99% min
Quick product specifications
2,4-Dinitro Fluoro Benzene at a glance
Identifiers, physical data and trade codes for 2,4-DNFB. Batch-specific figures are confirmed on the Certificate of Analysis supplied with each consignment, in India and on export shipments alike.
- Chemical name
- 2,4-Dinitro Fluoro Benzene
- CAS number
- 70-34-8
- Molecular formula
- C6H3FN2O4
- Molecular weight
- 186.10 g/mol
- IUPAC name
- 1-Fluoro-2,4-dinitrobenzene
- EC number
- 200-734-3
- HS code
- 2904 99National subheading as per destination
- UN number
- UN 2811Toxic solid, organic, n.o.s. · Class 6.1, PG II. Shipped under the liquid entry when despatched above its melting point
- Appearance
- Yellow crystalline solidMelts just above room temperature; may arrive as a yellow melt in warm weather
- Melting point
- 25–27 °C
- Boiling point
- About 296 °C
- Density
- About 1.48 g/cm³At 25 °C
- Flash point
- Above 110 °CClosed cup, combustible
- Solubility
- Practically insoluble in waterSoluble in ethanol, ether, benzene and propylene glycol; hydrolyses slowly in water
- Purity
- 99% min
- Synonyms
- 2,4-DNFBDNFB · 1-Fluoro-2,4-dinitrobenzene · Sanger's reagent · FDNB
- Product form
- Crystals or melt
- Documentation
- COA, MSDS & TDSSupplied with every order
Product overview
What is 2,4-Dinitro Fluoro Benzene?
2,4-Dinitro Fluoro Benzene (2,4-DNFB) is a fluorinated nitroaromatic in which two nitro groups activate the ring fluorine so strongly that it is displaced by amines, alcohols and thiols under mild conditions. That reactivity is what made it famous as Sanger's reagent for identifying the N-terminal amino acid of a peptide.
With the formula C6H3FN2O4 (CAS 70-34-8), 2,4-DNFB is a yellow crystalline solid that melts at about 26 °C — so it is often received as a yellow melt in warm weather — and is practically insoluble in water, though it hydrolyses slowly on contact with moisture. It is made from 1-chloro-2,4-dinitrobenzene by halogen exchange with a fluoride salt.
Beyond protein chemistry it is used wherever a 2,4-dinitrophenyl group needs to be attached cleanly: as a derivatising reagent in analysis, as an intermediate for pharmaceutical and dye chemistry, and as the standard sensitiser in immunology research. Because the ring fluorine is so labile, material must be kept dry and away from amines and bases in storage.
How it is made
2,4-Dinitrofluorobenzene is made from 1-chloro-2,4-dinitrobenzene by halogen exchange (halex) with potassium fluoride in a polar aprotic solvent. The crude product is washed, dried and purified by vacuum distillation or crystallisation, then packed under dry conditions and protected from light.
What that means for your process
- Highly activated ring fluorine for mild, selective substitution
- Low residual chloro-analogue and free fluoride, checked by GC
- Low moisture to prevent hydrolysis to 2,4-dinitrophenol
- Light-protected packing for analytical-grade consistency
Applications
What is 2,4-Dinitro Fluoro Benzene used for?
2,4-DNFB is used both as a laboratory reagent and as a synthetic intermediate. Its main applications, industry by industry, include:
Protein & peptide analysis
The classic Sanger's reagent: it labels the free alpha-amino group so the N-terminal residue can be identified after hydrolysis.
- N-terminal end-group determination
- DNP-amino acid derivatives for chromatography
- Quantifying free amino groups in peptides
Pharmaceutical intermediates
A clean way to install a 2,4-dinitrophenyl group or to build fluorinated nitroaromatic scaffolds.
- Nucleophilic aromatic substitution with amines and thiols
- Fluoroaromatic building blocks for API synthesis
- Protecting and derivatising reagent in fine chemistry
Dyes, pigments & research reagents
Used for dinitrophenyl colourants and as the standard hapten in contact-sensitisation research.
- 2,4-Dinitrophenyl dye and pigment intermediates
- Reference hapten in immunology studies
- Analytical derivatisation reagent
Process route
How is 2,4-DNFB used in end-group analysis and synthesis?
In both laboratory and plant use the chemistry is the same: nucleophilic aromatic substitution. The two nitro groups pull electron density away from the ring, the fluorine leaves, and the nucleophile ends up carrying a 2,4-dinitrophenyl group that is stable to acid hydrolysis — which is exactly what makes N-terminal analysis work.
2,4-Dinitro Fluoro Benzene
Charged dry, under mild alkaline conditions. Assay and moisture decide conversion.
C6H3FN2O4Nucleophilic substitution
The amine, alcohol or thiol displaces the activated ring fluorine at mild temperature.
SNAr2,4-Dinitrophenyl derivative
The DNP-labelled product is isolated; the label survives acid hydrolysis.
DNP derivativeAnalysis or onward synthesis
Identified chromatographically in end-group work, or carried forward as an API or dye intermediate.
DNP-amino acidIn depth
2,4-Dinitro Fluoro Benzene applications by industry
Below is a detailed breakdown of where 2,4-DNFB is used and the role it plays in each downstream product.
| Industry | Typical downstream products | Role of 2,4-DNFB |
|---|---|---|
| Life sciences & analysis | Sanger's end-group analysis, DNP-amino acids | Derivatising reagent |
| Pharmaceuticals | Fluoroaromatic and dinitrophenyl API intermediates | Intermediate |
| Dyes & pigments | 2,4-Dinitrophenyl colourant intermediates | Starting material |
| Research reagents | Reference hapten for contact-sensitisation studies | Reagent |
| Fine chemicals | Custom nitro- and fluoro-aromatics | Building block |
Safety
Is 2,4-Dinitro Fluoro Benzene hazardous?
Yes, and it deserves particular respect. 2,4-DNFB is toxic if swallowed, in contact with skin or if inhaled, causes severe skin burns and eye damage, and is a potent skin sensitiser — it is used in research precisely because it reliably provokes allergic contact dermatitis. It is also harmful to aquatic life.
2,4-DNFB should be stored in sealed containers in a cool, dry, ventilated place, protected from light and moisture and kept well away from amines, ammonia, strong bases and reducing agents, with which it reacts readily. Handle it only with chemical-resistant gloves (changed frequently), goggles or a face shield, impervious protective clothing and respiratory protection, inside a fume hood or under local exhaust ventilation. Once a person is sensitised, even trace contact can provoke a reaction, so contamination control is as important as the PPE itself. A full MSDS is supplied with every order.
Signal word: Danger
Toxic by ingestion, skin contact and inhalation · causes severe skin burns and eye damage · may cause an allergic skin reaction · harmful to aquatic life
Safe-handling essentials
- Handle in a fume hood or under local exhaust ventilation
- Chemical-resistant gloves changed frequently; double-glove for transfers
- Goggles or face shield and impervious protective clothing
- Treat as a strong sensitiser — avoid even trace skin contact
- Keep containers sealed, dry and protected from light
- Store well away from amines, ammonia, strong bases and reducing agents
- Keep away from moisture — it hydrolyses to 2,4-dinitrophenol
- Transport as UN 2811, class 6.1, packing group II
Grades
2,4-Dinitro Fluoro Benzene grades & technical specifications
We supply 2,4-DNFB in technical grade for synthesis and in high-purity, low-moisture reagent grade for analytical and biochemical work.
| Parameter | Specification |
|---|---|
| Assay (purity) | 99% min |
| Appearance | Yellow crystals or yellow melt |
| 1-Chloro-2,4-dinitrobenzene | 0.5% max |
| 2,4-Dinitrophenol | 0.3% max |
| Setting point | 24.5 °C min |
| Moisture | 0.1% max |
| Acidity (as HF) | 0.05% max |
| Product form | Crystals or melt |
Note: Values are representative. Batch-specific figures are provided on the Certificate of Analysis (COA) supplied with each consignment.
Supply
Packaging, MOQ & supply
From trial quantities for process validation to repeat container loads on contract — packed, documented and dispatched to your plant in India or overseas.
Packaging
25 kg or 50 kg HDPE drums and light-proof fibre drums; smaller lab packs for analytical and R&D use on request.
Minimum order quantity
Flexible — laboratory packs for method development through to drum and pallet quantities.
Shelf life
Good shelf life in sealed, unopened packs stored cool, dry and protected from light and moisture.
Dispatch
Pan-India dispatch and worldwide export with complete dangerous-goods documentation and logistics support.
Why buy from us
Why choose Chelora as your 2,4-DNFB supplier?
As an established 2,4-Dinitro Fluoro Benzene supplier and distributor based in India and shipping worldwide, we work with a wide network of trusted manufacturers to combine consistent quality with dependable service. More about Chelora Agrochem.
High purity, every batch
99% min assay, sourced only from quality-approved manufacturers we have worked with and audited.
Full documentation
COA, MSDS and TDS travel with every order, so your QA and EHS teams are never chasing paperwork.
Reliable bulk supply, flexible MOQ
Single packs for process trials through to repeat container loads on schedule.
Worldwide supply
Export-grade packing, dangerous-goods documentation and on-time dispatch to plants across Asia, the Middle East, Europe, Africa and the Americas.
Technical & safe-handling support
Guidance on storage, PPE, sensitisation control and process fit from a team that knows fluorinated nitroaromatics.
One point of contact
Source 2,4-DNFB alongside your other intermediates — one enquiry, one team, one invoice.
Ordering
How to order 2,4-Dinitro Fluoro Benzene from Chelora
Ordering is simple and fast — most enquiries are answered with pricing and availability within 24 hours.
- 1
Send your enquiry
Tell us the grade, quantity and application you are buying for.
- 2
Receive a quotation
We send pricing along with the COA and MSDS for your review.
- 3
Request a sample
Test suitability for your process before you commit to a bulk order.
- 4
Confirm the order
Lock in quantity, packaging and delivery preference.
- 5
Dispatch & tracking
We dispatch with full documentation and keep you updated until delivery.
Documentation
2,4-Dinitro Fluoro Benzene MSDS & COA
A summary of key safety information and a representative Certificate of Analysis. The complete 16-section Safety Data Sheet and the actual batch COA are supplied with each shipment — and both are sent with your quotation when you ask for a quote.
Safety data summary (MSDS)
| Section | Details |
|---|---|
| Hazard classification | Acute toxicity cat. 3 (oral / dermal / inhalation); skin corrosion cat. 1B; serious eye damage cat. 1; skin sensitisation cat. 1; aquatic chronic cat. 2 |
| Signal word | Danger |
| First aid — inhalation | Move to fresh air; keep at rest; get immediate medical attention |
| First aid — skin | Remove contaminated clothing at once; wash with plenty of soap & water; get immediate medical attention |
| First aid — eyes | Rinse cautiously with water for several minutes; get immediate medical attention |
| Handling | Fume hood or local exhaust; full PPE; avoid all skin contact; strong sensitiser |
| Storage | Cool, dry, sealed and protected from light; away from amines, bases & reducing agents |
| Environment | Harmful to aquatic life with long-lasting effects; prevent release to drains / waterways |
| Transport | UN 2811; class 6.1; packing group II |
Certificate of Analysis (representative)
| Test parameter | Specification | Method |
|---|---|---|
| Assay (purity) | 99% min | GC |
| Appearance | Yellow crystals | Visual |
| Chloro analogue | 0.5% max | GC |
| 2,4-Dinitrophenol | 0.3% max | HPLC |
| Setting point | 24.5 °C min | Capillary |
| Moisture | 0.1% max | Karl Fischer |
The actual batch COA is supplied with each shipment.
FAQ
Frequently asked questions
Common questions from buyers specifying 2,4-DNFB for pharmaceutical synthesis, dye chemistry and laboratory analysis.
What is 2,4-Dinitro Fluoro Benzene used for?
2,4-DNFB is used as Sanger's reagent for identifying the N-terminal amino acid of peptides and proteins, as a derivatising reagent in analysis, as an intermediate for fluoroaromatic and dinitrophenyl pharmaceutical and dye chemistry, and as a reference hapten in contact-sensitisation research.
What is the CAS number of 2,4-DNFB?
The CAS number is 70-34-8 and the molecular formula is C6H3FN2O4, with a molecular weight of 186.10 g/mol. It is also known as 1-fluoro-2,4-dinitrobenzene, FDNB or Sanger's reagent.
Why is it called Sanger's reagent?
Frederick Sanger used it to label the free amino group at the N-terminus of a peptide chain. The resulting dinitrophenyl label survives the acid hydrolysis that breaks the chain into individual amino acids, so the labelled residue can be identified chromatographically — the method he used in determining the structure of insulin.
How is 2,4-Dinitro Fluoro Benzene made?
It is made from 1-chloro-2,4-dinitrobenzene by halogen exchange with potassium fluoride in a polar aprotic solvent, then purified by vacuum distillation or crystallisation and packed dry and protected from light.
Is 2,4-Dinitro Fluoro Benzene hazardous?
Yes. It is toxic if swallowed, in contact with skin or if inhaled, causes severe skin burns and eye damage, and is a potent skin sensitiser that can cause allergic contact dermatitis. Handle it only with full PPE in a fume hood or under local exhaust ventilation; we supply a full MSDS with every order.
How should 2,4-DNFB be stored?
In sealed containers in a cool, dry, well-ventilated store, protected from light and moisture and kept well away from amines, ammonia, strong bases and reducing agents. Moisture hydrolyses it slowly to 2,4-dinitrophenol, so containers should be resealed promptly after use.
Do you provide COA and MSDS for 2,4-DNFB?
Yes, we provide a batch-specific COA and MSDS with every order, plus technical support on request.
Do you supply and export 2,4-Dinitro Fluoro Benzene?
Yes, we supply 2,4-DNFB across India and export internationally with complete dangerous-goods documentation, sourced from reliable manufacturers.
Request a quote or sample
Need a reliable 2,4-DNFB supplier?
Looking for a dependable 2,4-Dinitro Fluoro Benzene (2,4-DNFB) supplier and distributor — in India or anywhere we export to? Tell us the grade and quantity you need — our team responds with competitive bulk pricing, samples and complete documentation (COA, MSDS, TDS) within 24 hours.
Or email source@cheloraagrochem.com